The effect of ozone therapy on experimental bone fracture healing in rats

1Ozone therapist and Pain Specialist, Medipol University, Medical School, Department of Anesthesiology and Reanimation, Medipol Mega University Hospital Bagcilar, Istanbul, Turkey. 2Histology and Embryology, Acibadem University, Vocational School of Health Services, Istanbul, Turkey 3Acibadem University Medical School, Department of Orthopedic and Traumatology, Istanbul, Turkey 4Acibadem University Medical School, Department of Neurology, Istanbul, Turkey 5Acibadem University, Vocational School of Health Services, Istanbul, Turkey 6Ozone therapist and Pain Specialist, Emsey Hospital, Istanbul, Turkey 7Acibadem University Medical School, Department of Physiology, Istanbul, Turkey Ozone has been used as a therapeutical agent for a long time. In the literature, many beneficial effects are attributed ozone; it balances the oxidant-antioxidant system, leads to delivery of super enriched oxygen at a cellular level and optimizes cell function, etc. Previously, beneficial effect of ozone on cutaneous wound healing was shown in experimental model in mice.The aim of this study was to evaluate if there is a positive impact of ozone therapy on bone healing in an experimental fracture model in rats. After approval of experimental animal ethic committee, an open femoral fracture (ffx) was performed in 48 Spraque-Dawley male rats. They were randomly divided into two groups: in Group O, medical ozone/oxygen mixture (500mcg/kg) and in Group C, medical air in volume of 4 mL were given rectally for 3-consequtive days. At days 4, 7 and 13, eight rats from each group were euthanized, and histomorphometric and immunohistochemical bone tissue evaluation was undertaken from the femur sampling. Histomorphometrically, periost thickness was thicker, trabecular areas were larger, cartilaginous and new bone areas were smaller (p<0.05 for each) and TGF-β expressions were more intense in Group O(p<0.001 for all times). Immunohistochemically, a significant increase in VEGF expressions in day 7 and 13 (p<0.001 for each) and higher β-catenin expressions were observed in Group O at day 7 and 13 (p<0.001 for each).The current data indicate that ozone therapy could have positive impacts on bone healing process.